TABLE 1

Effect of DAMGO pretreatment on DAMGO-stimulated [35S] GTPγS binding in HμOR and HμOR-mPKCI expressing cells

HμCHO HμCHO-mPKCI
Maximal Response EC50 Maximal Response EC50
% nM % nM
Without pretreatment of DAMGO 224.0 ± 6.2 9.3 ± 0.8 182.0 ± 4.8 10.5 ± 0.8
With pretreatment of DAMGO 168.0 ± 4.2# 68.9 ± 0.8## 141.0 ± 2.9# 36.8 ± 0.8#*

  • ↵# p < 0.05

  • ↵## p < 0.01, compared with that without DAMGO pretreatment

  • ↵* p < 0.05, compared with that in HμCHO cells