TABLE 2

Effect of DAMGO pretreatment on HμOR-mediated inhibition of forskolin-stimulated adenylyl cyclase activity in cells expressing HμOR alone or with mPKCI

HμCHO HμCHO-mPKCI
Maximal Response EC50 Maximal Response EC50
% nM % nM
Without pretreatment of DAMGO 68.9 ± 2.9 2.6 ± 0.9 69.1 ± 2.3 10.2 ± 0.8**
With pretreatment of DAMGO 55.7 ± 0.3# 9.2 ± 0.6## 68.5 ± 0.4* 13.4 ± 0.6

  • ↵# p < 0.05

  • ↵## p < 0.01, compared with that without DAMGO pretreatment

  • ↵* p < 0.05

  • ↵** p < 0.01, compared with that in HμCHO cells