Cholesterol modification procedure Cholesterol alterations were carried out as described under Materials and Methods.
| Preincubation (15 min) | First incubation (15 min) | Second incubation (15 min) | |
|---|---|---|---|
| Control | HHBSS + BODIPY-verapamil (± P-glycoprotein inhibitora) | HHBSS + BODIPY-verapamil (± P-glycoprotein inhibitora) | |
| Depletion/repletion | HHBSS + BODIPY-verapamil (± P-glycoprotein inhibitora) | MβCD-HHBSS + BODIPY-verapamil (± P-glycoprotein inhibitora) | Cholesterol/MβCD-HHBSS + BODIPY-verapamil (± P-glycoprotein inhibitora) |
| Saturation | HHBSS + BODIPY-verapamil (± P-glycoprotein inhibitora) | Cholesterol/MβCD-HHBSS + BODIPY-verapamil (± P-glycoprotein inhibitora) |
↵ a P-glycoprotein inhibitor was 10 μM of either LY335979 or SDZ-PSC833