EC50 values for different organic nitrates and the endothelium-dependent vasodilator ACh in the presence and absence of the ALDH-2 inhibitor benomyl. Vessels were preincubated in the absence or presence of 10 μM benomyl for 30 min.
| Condition | EC50 without Benomyl | EC50 with Benomyl |
|---|---|---|
| GTN | −7.66 ± 0.08 (n = 19) | −6.56 ± 0.13*** (n = 10) |
| GTN upon GTN in vivoa | −6.54 ± 0.25††† (n = 10) | − 6.27 ± 0.08 (n = 4) |
| PETN | −8.07 ± 0.06 (n = 10) | −7.16 ± 0.06*** (n = 6) |
| PETN upon 100 μM PETNb | −7.57 ± 0.13# (n = 6) | N.D. |
| PETN upon 100 μM GTNb | −7.07 ± 0.09### (n = 5) | N.D. |
| PETN upon GTN in vivoa | −6.74 ± 0.05### (n = 4) | N.D. |
| PETriN | −6.63 ± 0.11 (n = 6) | −5.76 ± 0.16* (n = 6) |
| ISDN | −5.29 ± 0.04 (n = 12) | −5.28 ± 0.23 (n = 7) |
| PEDN | −4.61 ± 0.13 (n = 6) | −4.66 ± 0.06 (n = 6) |
| ISMN | −3.77 ± 0.04 (n = 7) | −3.51 ± 0.14 (n = 6) |
| PEMN | −2.97 ± 0.18 (n = 4) | −3.10 ± 0.11 (n = 4) |
| ACh | −7.46 ± 0.07 (n = 55) | −7.47 ± 0.08 (n = 4) |
N.D., not determined.
*P < 0.05, ***P < 0.001; significance versus sample without benomyl treatment.
†††P < 0.001; significance versus relaxation of control in response to GTN.
#P < 0.05, ### P < 0.001; significance versus relaxation of control in response to PETN.
↵ a Dose-response curves recorded with tolerant vessels from rats that were treated for 3 days with GTN (0.48 μmol/h/kg).
↵ b Dose-response curves recorded with vessels that were treated in vitro with either 100 μM PETN or GTN for 30 min at 37°C.