TABLE 1

Effect of AM-251 (1 μM), AM-630 (1 μM), and capsazepine (1 μM) on R(+)-MA-induced accumulation of cytoplasmic DNA fragments and subsequent death of H4 cells

Cells were incubated with the indicated substances, R(+)-MA (10 μM), or its vehicles for 48 h (DNA fragmentation) or 72 h (cell viability). Receptor antagonists were added 1 h before R(+)-MA. Percent control represents comparison with vehicle-treated cells (100%) in the absence of test substances. Percent control represents comparison with vehicle-treated cells (100%) in the absence of test substance. Values are means ± S.E.M. of n = 3 (DNA fragmentation) or n = 4 (cell viability) experiments.

DNA Fragmentation Cell Viability
%
Vehicle 100 ± 5 100 ± 14
R(+)-MA 204 ± 14 59 ± 5
R(+)-MA + AM-251 173 ± 5 55 ± 7
R(+)-MA + AM-630 206 ± 11 59 ± 9
R(+)-MA + AM-251 + AM-630 182 ± 22 52 ± 11
R(+)-MA + capsazepine 251 ± 13 58 ± 2