TABLE 1

Binding of epibatidine to desensitized full pentameric receptors containing all mouse subunits The parameters [dissociation constants K₁ and K₂ and proportion (P) of sites with affinity K₁] are derived from fits of eq. 2 to our data and are expressed as mean ± S.E. Data are the mean of three to five experiments.

Wild-Type Subunit	Mutant Subunit	K₁	K₂	P
		nM
α₂βδγ		0.49 ± 0.04	201 ± 36	0.66 ± 0.03
α₂βδ+	γL104Y	0.84 ± 0.09	168 ± 60	0.62 ± 0.04
	γS111Y	0.66 ± 0.12	180 ± 18	0.42 ± 0.03
	γY117T	1.92 ± 0.04	184 ± 59	0.47 ± 0.05
α₂βγ+	δY106L	0.48 ± 0.1	59 ± 6.8	0.55 ± 0.06
	δY113S	0.36 ± 0.03	83 ± 11	0.534 ± 0.09
	δT119Y	0.61 ± 0.2	71 ± 25	0.58 ± 0.1