Effect of AH 6809 and L-161,982 alone and in combination on the EC50 values for 8-iso-PGE1- and 8-iso-PGE2-induced G-CSF release
Values in parentheses indicate number of determinations. Adherent HASM cells were pretreated (30 min) with AH 6809 (10 μM) or L-191,982 (2 μM) alone and in combination and then for a further 5 min with 8-iso-PGE1 and 8-iso-PGE2. IL-1β (10 pg/ml) was then added to the cells, and the G-CSF released into the culture medium was quantified at 24 h by a sandwich ELISA. See text for further details.
| Treatment | -log EC50 | |
|---|---|---|
| 8-iso-PGE1 | 8-iso-PGE2 | |
| M | ||
| Vehicle | 6.53 ± 0.19 (9) | 6.89 ± 0.51 (6) |
| AH 6809 (10 μM) | 6.67 ± 0.24 (9) | 7.27 ± 0.49 (6) |
| Vehicle | 6.30 ± 0.13 (8) | 6.37 ± 0.16 (8) |
| L-161, 982 (2 μM) | 6.11 ± 0.13 (8) | 6.27 ± 0.21 (8) |
| AH 6809 | 6.48 ± 0.14 (8) | 6.58 ± 0.12 (7) |
| AH 6809 + L-161,982 | 5.98 ± 0.11* (8) | 6.04 ± 0.09* (7) |
| L-161,982 | 6.57 ± 0.11 (8) | 6.51 ± 0.11 (7) |
| L-161,982 + AH 6809 | 5.98 ± 0.10* (8) | 6.13 ± 0.12* (7) |
↵* P < 0.05, significant difference in -log EC50 values with respect to vehicle or single antagonist-treated cells.