Quantity of [3H]MC-DNA adducts formed by parental and NPR-transfected cell lines with the enzyme localized to either the nucleus or the endoplasmic reticulum treated under aerobic and hypoxic conditions
[3H]MC-DNA was isolated from cells treated with 10 μ M [3H]MC for 2 h as described under Materials and Methods. Values shown are the means of three to four determinations ± S.E. Significant differences between groups were determined using the paired Student's t test. There were no significant differences between the values for any cell line treated under aerobic conditions. Significant differences are found for comparisons marked a to e.
| [3H]MC-DNA adducts | ||
|---|---|---|
| Aerobic | Hypoxic | |
| cpm / μg of DNA | ||
| CHO Parental | 0.37 ± 0.01 | 0.78 ± 0.01a |
| CHO-NPR-16 | 0.40 ± 0.04 | 1.05 ± 0.01a,b |
| CHO-NLS-NPR-9A | 0.40 ± 0.02 | 1.20 ± 0.03a,c,d |
| CHO-NLS-NPR-13A | 0.39 ± 0.01 | 1.69 ± 0.10a,d,e |
↵ a Values are significantly different from aerobic counterparts, P < 0.0001.
↵ b Values are significantly different from the parental cells, P = 0.0001.
↵ c Values are significantly different from the parental cells, P < 0.001.
↵ d Values are significantly different from ER-NPR, P < 0.05.
↵ e Values are significantly different from the parental cells, P < 0.02.