Effects of various compounds on [3H]DHEAS transport
Membrane vesicles prepared from LLC/PK1-MRP8-3 cells were incubated at 37° C for 10 min in media containing 100 nM [3H]DHEAS in the presence of the indicated compounds. ATP-dependent uptake was calculated by subtracting values obtained in the presence of 4 mM ATP from those in the presence of 4 mM AMP. Transport is expressed as percentage uptake in the absence of modulator. Values shown are means ± S.E. of at least three measurements performed in duplicate.
| Compound | Concentration | Uptake |
|---|---|---|
| μM | % of control | |
| Control | 100 | |
| cAMP | 10 | 86.2 ± 3.7 |
| cGMP | 10 | 82.3 ± 6.7 |
| LTC4 | 1 | 80.2 ± 5.3 |
| DNP-SG | 1 | 92.4 ± 6.8 |
| DNP-SG | 10 | 56.3 ± 8.9 |
| MTX | 10 | 77.5 ± 11 |
| MTX-Glu2 | 10 | 96.4 ± 5.3 |
| MTX-Glu3 | 10 | 95.5 ± 10 |
| GC | 10 | 65.7 ± 4.6 |
| TC | 10 | 66.6 ± 1.7 |
| GLC | 10 | 29.0 ± 14 |
| E217βG | 10 | 80.3 ± 11 |
| Sildenafil | 10 | 82.4 ± 5.4 |
| MK571 | 1 | 85.6 ± 9.3 |
| MK571 | 10 | 21.6 ± 15 |
| Probenecid | 10 | 91.4 ± 8.9 |
| Indomethacin | 10 | 76.4 ± 2.2 |
| Sulfanitran | 10 | 101 ± 10 |
| Sulfinpyrazone | 10 | 92.1 ± 5.6 |
| Penicillin G | 10 | 98.3 ± 7.0 |