AC VI NT (1-163 aa) | |
Forward | (ACF2) 5′-TCAAGCTT(HindIII)ATGGACTACAAGGACGACGAT-3′ |
Reverse | 5′-GCTCTAGA(XbaI)CTAGCTGCTCTGGTTCATCTGGAAG-3′ |
AC VI TM1T (1-319 aa) | |
Forward | (ACF2)5′-TCAAGCTT(HindIII)ATGGACTACAAGGACGACGAT-3′ |
Reverse | 5′-GCTCTAGA(XbaI)CTAGTGCGTGCAGACACCGATGGCAT-3′ |
AC VI 547T (1-547 aa) | |
Forward | (ACF2)5′-TCAAGCTT(HindIII)ATGGACTACAAGGACGACGAT-3′ |
Reverse | 5′-GCTCTAGA(XbaI)CTAACGGCCTGGCTCCACCTCATAG-3′ |
AC VI C1aT (320-547 aa) | |
Forward | 5′-CCCAAGCTT(HindIII)GCCACCATGGATTACAAGGATGACGACGATAAGTACCCCGCTGAAGTGTCTCAG-3′ |
Reverse | 5′-GCTCTAGA(XbaI)CTAACGGCCTGGCTCCACCTCATAG-3′ |
AC VI TM2T (548-1181 aa) | |
Forward | 5′-CCCAAGCTT(HindIII)GCCACCATGGATTACAAGGATGACGACGATAAG GGC GGTGAG CGCAACGCGTAC-3′ |
Reverse | 5′-GCTCTAGA(XbaI)CTAACTGCTGGGGCCCCCATTGAG-3′ |
AC I 462T (1-462 aa) | |
Forward | 5′-CGGGATCC(BamHI)GCCACCATGGATTACAAGGATGACGAC-3′ |
Reverse | 5′-GCTCTAGA(XbaI)CTAGTGTCCGTGTCCCGGCTCCACCT-3′ |
fp, forward primer; rp, reverse primer.