ChIP-PCR primer sequences and amplification settings
| Gene/Clone | Primer Name | Primer Direction | Primer Sequence | Fragment Length | Annealing Temp. |
|---|---|---|---|---|---|
| bp | °C | ||||
| HNF1α | Ni16 | Forward | CATGATGCCCCTACAAGGTT | 274 | 60 |
| Reverse | ATTGGAGCTGGGGAAATTCT | ||||
| ApoB | Ni56 | Forward | GAGGCTCTTCAAGGCTCAAA | 181 | 60 |
| Reverse | AACCGAGAAGGGCACTCAG | ||||
| AAT | Ni75 | Forward | GGAACAGCCACTAAGGATTCTGCAGTGAG | 380 | 60 |
| Reverse | TCACTGTCCCAGGTCAGTGGTGGTGCCTG | ||||
| ANG | Ni117 | Forward | GGGAGATGTACCCCCAAGAG | 200 | 60 |
| Reverse | GCTTGTGTGTTTTCCCCAGT | ||||
| Clone 23, prosite a | Ni8 | Forward | CCAACTCAGGACCTTGGAGA | 141 | 60 |
| Reverse | GGCCAGCTTTGCTTCATTAG | ||||
| Clone 23, prosite b | Ni13 | Forward | GAGCTGCTGTGCCTGGTACT | 148 | 60 |
| Reverse | TTTTTGCTGACGGGAGAGAT | ||||
| Clone 113, site a | Ni61 | Forward | TCATCACGGACATAAAGATGGA | 160 | 60 |
| Reverse | GCATAGTGGTGGGGGTTCT | ||||
| Clone 113, site b | Ni73 | Forward | AGCAGAACCCCCACCACTAT | 187 | 60 |
| Reverse | TCACCCAGAAAGTTCCCTTG | ||||
| Clone 113, prosite a | Ni5 | Forward | CCGGTCAAGTCTGAACCAGT | 126 | 60 |
| Reverse | TTAACTAGGCAAGCCCAAGC | ||||
| Clone 113, prosite c | Ni116 | Forward | TAGTCCCTGTGGCTGCAGTA | 232 | 60 |
| Reverse | TCTCAATGGCTGATTACAGGTT |