TABLE 1

¹²⁵I-PYY saturation and competition binding in stably transfected CHO HAY1, HAY1T*, and HAY1S* clones

pK_d and B_max values were calculated from the direct fit to saturation experiments performed with 0.05 to 5.0 nM ¹²⁵I-PYY, in which nonspecific binding was assessed with 1 μM BIBO3304. PYY, NPY, and BIBO3304 pIC₅₀ values are given for competition assays performed with 10 pM radioligand; 1 μM GTPγS attenuated total ¹²⁵I-PYY binding (expressed as percentage inhibition of total binding) without altering the pIC₅₀ values for competing PYY significantly (range, 9.22-9.57). Data are expressed as mean ± S.E.M. from the number of experiments indicated in parentheses.