Summary of functional responses in colony 1 epithelial clones
The peak changes in ISC in response to VIP, PYY, and the α2-adrenoceptor agonist UK14,304 are indicated for nontransfected colony 1 epithelial layers and the three clones expressing Y1, Y1(S352*), and Y1(T361*) receptors. Data are expressed as mean ± S.E.M., with n values indicated in parentheses.
| 30 nM VIP | 100 nM PYY | 1 μM UK14,304 | |
|---|---|---|---|
| μA/cm2 | |||
| Colony 1 | +29.6 ± 1.2 (200) | 0.0 ± 0.0 (21) | -10.4 ± 0.9 (4) |
| C1Y1 | +34.9 ± 1.8 (69) | -9.4 ± 1.2 (8) | -26.0 ± 6.0 (4) |
| C1Y1S* | +26.4 ± 1.8 (54) | -5.4 ± 0.9 (5) | -8.5 ± 2.1 (4) |
| C1Y1T* | +48.5 ± 2.3 (53) | -5.2 ± 0.8 (8) | -8.0 ± 1.8 (3) |