TABLE 2

Reduced gene transcription after TPA + dexamethasone treatment compared with TPA alone (2 h)

Genes shown in bold were validated by real-time PCR. Negative values mean transcriptional activation.

GenBank Accession No. Probe ID Gene Name % Repression
WT R488Q
Apoptosis
    NM_003897 201631_s_at IER3 71 15 •
    NM_016639 218368_s_at TNFRSF12A 37 1 •
Cell growth/cell cycle
    AK023795 222162_s_at ADAMTS1 55 -26 •
    NM_001554 201289_at CYR61 52 -15
    NM_002010 206404_at FGF9 58 23 •
    BC004490 209189_at FOS 40 7 •
    NM_006732 202768_at FOSB 87 38 •
    NM_005542 201625_s_at INSIG1 37 -3 •
    BE327172 213281_at JUN 41 17 •
    M24779 209193_at PIM1 32 -15 •
Inflammation/immune response
    NM_000963 204748_at COX-2 45 15 •
    NM_005261 204472_at GEM 79 41 ○
Regulation of transcription
    NM_001186 204194_at BACH1 46 25 ○
    NM_003670 201170_s_at BHLHB2 69 13 •
    NM_004405 207147_at DLX2 36 18 ○
    NM_004430 206115_at EGR3 60 17 •
    NM_001427 207060_at EN2 39 4 •
    NM_004496 204667_at FOXA1 44 5 ○
    U90304 210239_at IRX5 40 -13 •
    NM_002229 201473_at JUNB 70 31 •
    NM_005384 203574_at NFIL3 46 23 ○
    S77154 216248_s_at NR4A2 57 0 •
    NM_003107 201417_at SOX4 31 12 ○
    BF343007 204653_at TFAP2A 47 21 •
    NM_005655 202393_s_at TIEG 44 -3 •
Stress response
    NM_001924 203725_at GADD45A 38 24 ○
    NM_015675 207574_s_at GADD45B 48 38 ○
    NM_005904 204790_at MADH7 62 19 •
Other genes
    AF127481 209535_s_at AKAP13 41 -15 •
    NM_016201 203002_at AMOTL2 33 13 ○
    NM_025195 202241_at C8FW 65 -6 •
    NM_004907 202081_at ETR101 45 10 •
    NM_005242 206429_at F2RL1 35 1 •
    NM_001450 202949_s_at FHL2 42 16 ○
    U41813 214651_s_at HOXA9 42 17 •
    NM_000527 202068_s_at LDLR 52 15 •
    NM_014575 204030_s_at SCHIP1 37 6 •
    AL574096 209277_at TFPI2 64 29 •
    NM_020127 205807_s_at TUFT1 31 8 •

  • ○, no discrimination; •, discrimination; WT, wild type. Some genes were scored as nondiscriminating, although repression by the GRR488Q was less than 50% compared with the repression by the GRwt, because they did not fulfill the statistical requirement (p < 0.01).