TABLE 1

Binding parameters of [³H]DAMGO, ¹²⁵I-deltorphin-I (¹²⁵I-Delt-I) and ¹²⁵I-EYF on membrane preparation from wild-type SH-SY5Y and hNPFF₂ receptor-transfected SH₂-D9 cells (control)

In SH₂-D9 cells, binding experiments also were performed on membranes preincubated (1DMe) for 20 min at room temperature with 1 μM 1DMe. Data are means ± S.E.M. of three to four experiments performed in duplicate.