TABLE 2

Relative binding, E_max, and EC₅₀ values of various GnRH receptors

Values represent the mean ± S.E.M. of three independent experiments performed in duplicate. Binding was expressed as a percentage of specific binding of gmGnRHR-2 (gm2).

Receptors	Relative Binding	GnRH-I		GnRH-II
Receptors	Relative Binding	E_max	EC₅₀	E_max	EC₅₀
	% gm2	fold induction	log M	fold induction	log M
gm2	100 ± 1.2	9.33 ± 0.35	−6.00 ± 0.10	13.82 ± 0.35	−8.54 ± 0.13
rat	98.8 ± 1.8	12.49 ± 0.26	−8.31 ± 0.06^*	8.78 ± 0.29	−7.54 ± 0.07^*
r6TM	89.5 ± 1.1	9.59 ± 0.33	−7.16 ± 0.12^*	5.45 ± 0.20	−7.95 ± 0.12
r6.5TM	168.1 ± 4.5	17.14 ± 0.38	−9.18 ± 0.08^*	17.53 ± 0.35	−8.48 ± 0.07
rEL3S	21.8 ± 0.1	5.79 ± 0.18	−6.64 ± 0.10	7.72 ± 0.21	−8.21 ± 0.09
rEL3L	110.1 ± 5.4	6.24 ± 0.14	−8.38 ± 0.09^*	6.37 ± 0.08	−8.76 ± 0.05
rEL3L/F^7.37I	110.3 ± 9.4	6.83 ± 0.31	−8.25 ± 0.16^*	10.77 ± 0.53	−8.24 ± 0.18
rEL3L/F^7.38L	138.7 ± 1.1	7.63 ± 0.35	−7.98 ± 0.18^*	10.56 ± 0.29	−8.34 ± 0.10
rEL3L/A^7.42G	49.7 ± 3.4	6.31 ± 0.22	−8.25 ± 0.09^*	5.43 ± 0.12	−8.79 ± 0.10
rEL3L/F^7.43L	52.7 ± 0.1	3.89 ± 0.27	−7.81 ± 0.20^*	4.61 ± 0.19	−8.63 ± 0.18
rEL3L/P^7.46A	3.8 ± 0.5	N.D.
rEL3L/C^7.47P	1.5 ± 1.0	N.D.
rEL3L/E^7.32Q	70.7 ± 0.2	3.53 ± 0.14	−7.28 ± 0.15^*	4.61 ± 0.14	−8.47 ± 0.14
rEL3L/PPS	332.2 ± 7.3	12.50 ± 0.27	−7.61 ± 0.07^*	16.78 ± 0.35	−9.36 ± 0.09^*
L^7.43F	1.7 ± 1.2	N.D.
A^7.46P	3.8 ± 0.9	N.D.
P^7.47C	72.1 ± 2.1	6.46 ± 0.26	−5.93 ± 0.08	12.29 ± 0.57	−8.29 ± 0.17
L^7.43F/A^7.46P	3.2 ± 2.8	N.D.
L^7.43F/P^7.47C	16.8 ± 1.2	3.57 ± 0.07	−7.03 ± 0.07^*	4.94 ± 0.15	−8.79 ± 0.17
A^7.46P/P^7.47C	303.3 ± 1.4	10.53 ± 0.61	−7.17 ± 0.17^*	11.00 ± 0.29	−9.65 ± 0.12^*
L^7.43F/A^7.46P/P^7.47C	178.6 ± 8.6	5.93 ± 0.20	−8.21 ± 0.13^*	7.06 ± 0.20	−9.82 ± 0.13^*
SEP/l^7.43F/A^7.46P/P^7.47C	11.1 ± 0.5	2.92 ± 0.08	−7.87 ± 0.12^*	2.53 ± 0.10	−9.69 ± 0.26^*
rEL3S/L^7.43F/A^7.46P/P^7.47C	54.6 ± 2.1	4.68 ± 0.13	−8.76 ± 0.15^*	6.14 ± 0.11	−9.59 ± 0.10^*
SEP/I^7.37F/L^7.38F/L^7.43F/A^7.46P/P^7.47C	51.2 ± 1.3	3.99 ± 0.14	−8.59 ± 0.17^*	4.76 ± 0.17	−9.32 ± 0.17^*
SEP/L^7.38F/L^7.43F/A^7.46P/P^7.47C	62.1 ± 1.7	5.27 ± 0.13	−8.79 ± 0.11^*	5.79 ± 0.14	−9.43 ± 0.11^*

N.D., not determined.
↵* P < 0.05 versus wild-type gm2.