Design of TAT-NSF polypeptides
All TAT-NSF fusion polypeptides contain the TAT domain YGRKKRRQRRR followed by a linker GGG followed by a unique NSF domain. The NSF domains were selected on the basis of studies of the structure and function of NSF.
| ID | Position | NSF Sequence | Rationale |
|---|---|---|---|
| TAT-NSF57 | 57-77 | GSVAFSLPQRKWAGLSIGQE | R67E SNAP binding |
| TAT-NSF81 | 81-101 | ALYSFDKAKQCIGTMTIEID | C91 NO Target |
| TAT-NSF222 | 222-243 | LDKEFNSIFRRAFASRVFPPE | Disassembly |
| TAT-NSF254 | 254-274 | KGILLYGPPGCGKTLLARQIG | C264 disassembly |
| Comatose mutant G260 | |||
| TAT-NSF700 | 700-720 | LLDYVPIGPRFSNLVLQALLVL | Hexamerization mutants |