Treatments used in generation gene expression profiles
Chemicals and physiological conditions that were used in generating the first phase of experiments for the EDGE database. Experiments listed are performed on C57Bl/6 male mice. The exception is the male liver RNA compared with female RNA. More detailed information regarding experimental conditions is available on the EDGE Web site at edge.oncology.wisc.edu
| Chemical | Route | Dose | Time | Mechanism |
|---|---|---|---|---|
| Acetominaphen | p.o. | 400 mg/kg | 1 day | Electrophile |
| TCDD | p.o. | 10 μg/kg | 2 days | AHR agonist |
| 3-Methylcholanthrene | i.p. | 100 mg/kg | 2 days | AHR agonist |
| β-Napthoflavone | p.o. | 100 mg/kg | 2 days | AHR agonist |
| Aroclor 1260 | i.p. | 200 mg/kg | 2 days | AHR agonist |
| Phenobarbital | p.o. | 100 mg/kg/day | 2 days | CAR agonist |
| TCPOBOP | i.p. | 3 mg/kg/day | 2 days | CAR agonist |
| Circadian rhythm | None | None | 0, 4, 8, 12, 16, 24 h | Circadian |
| Ketoconazole | i.p. | 250 mg/kg | 1, 4 days | P450 inducer |
| Cobalt | p.o. | 60 mg/kg | 2 days | Heme disrupter |
| Phenylhydrazine | p.o. | 100 mg/kg | 2 days | Heme disrupter |
| DEHP | Chow | 2% | 2 weeks | PPAR agonist |
| Ciprofibrate | Chow | 0.1% | 2 weeks | PPAR agonist |
| Wy-14,643 | Chow | 0.1% | 2 weeks | PPAR agonist |
| Corn oil | p.o. | 1 μl/g | 2 days | Vehicle |
| Corn oil | i.p. | 1 μl/g | 2 days | Vehicle |
| DMSO | i.p. | 1 μl/g | 2 days | Vehicle |
| Saline | p.o. | 1 μl/g | 2 days | Vehicle |
| Lipopolysaccharide | i.p. | 1 mg/kg | 6, 12, 24, 48 h | Inflammatory agent |
| Interleukin-1β | i.p. | 25 μg/kg | 6 h | Inflammatory response |
| Interleukin-6 | i.p. | 25 μg/kg | 6 h | Inflammatory response |
| Tumor necrosis factor-α | i.p. | 5 g/kg | 6, 12 h | Inflammatory response |
| Fasting | None | None | 1, 2 days | Metabolism |
| Sex | None | None | None | Sex |
AHR, aryl hydrocarbon receptor; DEHP, diethyl-hexyl-phthalate; TCPOBOP, 1,4-bis[2-(3,5-dichloropyridyloxy)]benzene.