Kinetic properties of hENT1 and hENT1-M33I
The Km, Vmax, and Ki values shown are the means ± S.E. of three to five separate experiments.
| Protein | Permeant | Km | Vmax | Vmax/Km | Ki |
|---|---|---|---|---|---|
| μM | pmol/mg/min | pmol/mg/min/μM | μM | ||
| hENT1 | Uridine | 44.1 ± 2.6 | 1060 ± 20 | 24.0 | 51.9 ± 2.8 |
| Cytidine | 234 ± 47 | 1280 ± 70 | 5.4 | 346 ± 49 | |
| Thymidine | N.D. | N.D. | N.D. | 81.6 ± 3.1 | |
| Adenosine | 17.8 ± 0.8 | 1330 ± 20 | 74.7 | 10.3 ± 0.4 | |
| Inosine | 28.5 ± 2.6 | 1300 ± 30 | 45.6 | 34.6 ± 1.9 | |
| Guanosine | 47.5 ± 4.8 | 1080 ± 30 | 22.7 | 48.6 ± 3.6 | |
| hENT1-M33I | Uridine | 30.0 ± 1.4 | 707 ± 10 | 23.6 | |
| Cytidine | 150 ± 38 | 814 ± 68 | 5.4 | ||
| Adenosine | 12.2 ± 0.5 | 1010 ± 10 | 82.8 | ||
| Inosine | 24.0 ± 4.0 | 804 ± 36 | 33.5 | ||
| Guanosine | 49.8 ± 4.7 | 784 ± 36 | 15.7 |
N.D., not determined.