Toxicity study of ShK(L5) Data are expressed as mean ± S.D.
| In vitro tests | 100 nM ShK(L5) |
|---|---|
| Cytotoxicity (% dead cells) | |
| Human PBMCs | 7.5 ± 4.3 |
| PAS T cells | 8.1 ± 0.8 |
| Jurkat cells | 5.5 ± 3.3 |
| Burkitt lymphoma | 3.1 ± 0.9 |
| RPMI 8226 myeloma | 6.5 ± 2.1 |
| Ames test | Negative |
| Acute in vivo tests | Saline | ShK(L5) 10 μg/kg |
|---|---|---|
| Electrocardiograma | ||
| Heart rate | 302 ± 13 | 311 ± 20 |
| SDNN | 13.3 ± 3.0 | 17.8 ± 4.4 |
| CV% | 6.7 ± 1.4 | 9.2 ± 2.2 |
| SDANN5 min | 5.0 ± 2.0 | 6.9 ± 2.3 |
| rMSSD | 6.8 ± 2.2 | 9.8 ± 3.5 |
| HF (n.u.) | 71 ± 21 | 79 ± 37 |
| HF (%) | 50 ± 8 | 53 ± 10 |
| LF (n.u.) | 68 ± 4 | 64 ± 10 |
| LF (%) | 50 ± 8 | 47 ± 10 |
| LF/HF | 1.1 ± 0.4 | 1.3 ± 0.7 |
| Subchronic in vivo tests | Saline | ShK(L5) 10 μg/kg/day for 2 weeks |
|---|---|---|
| Weight gain (%) | 7.2 ± 1.8 | 6.2 ± 1.7 |
| Complete blood count | ||
| Hematocrit (%) | 40.3 ± 1.4 | 39.0 ± 4.9 |
| Hemoglobin (g/dl) | 15.3 ± 0.5 | 15.0 ± 1.5 |
| MCV (fl) | 48.5 ± 0.2 | 48.3 ± 0.3 |
| MCH (pg) | 18.5 ± 0.8 | 18.5 ± 0.6 |
| MCHC (g/dl) | 38.0 ± 1.8 | 38.4 ± 1.3 |
| Total white cells (×103/mm3) | 7.1 ± 2.1 | 7.1 ± 2.5 |
| Total red cells (×106/mm3) | 8.3 ± 0.3 | 8.1 ± 1.0 |
| Total platelets (×103/mm3) | 656 ± 214 | 606 ± 106 |
| Blood chemistry | ||
| Alkaline phosphatase (U/L) | 170 ± 26 | 150 ± 18 |
| Glucose (mg/dl) | 139 ± 21 | 150 ± 18 |
| Blood urea nitrogen (mg/dl) | 17.1 ± 2.6 | 15.0 ± 1.7 |
| Creatinine (mg/dl) | 0.6 ± 0 | 0.6 ± 0.1 |
| Albumin (g/dl) | 5.0 ± 0.3 | 4.5 ± 0.4 |
| Thymic cell populations (%) | ||
| CD4− CD8− | 3.6 ± 1.1 | 4.3 ± 0.7 |
| CD4+ CD8+ | 77.8 ± 6.1 | 76.8 ± 4.1 |
| CD4+ CD8− | 8.5 ± 1.7 | 11.2 ± 2.0 |
| CD4− CD8+ | 10.0 ± 3.3 | 7.6 ± 1.3 |
| CD3+ | 89.5 ± 1.6 | 93.2 ± 3.5 |
| Splenic populations (%) | ||
| CD3+ | 72.4 ± 4.4 | 65.4 ± 0.1 |
| CD3+ CD45RC+ | 35.6 ± 2.6 | 39.8 ± 1.1 |
| CD3+ CD45RC− | 23.6 ± 2.3 | 26.5 ± 1.3 |
| CD3+ CD4+ | 62.7 ± 0.1 | 66.6 ± 1.2 |
| CD3+ CD8+ | 26.9 ± 0.1 | 25.0 ± 0.2 |
| IgM+ | 38.8 ± 1.5 | 33.3 ± 0.3 |
SDNN, standard deviation of all normal-to-normal RR intervals; CV%, 100 × SDNN/average RR interval; SDANN5 min, standard deviation of the mean of normal RR intervals for each 5-min period; rMSSD, root-mean-square of successive difference; HF (n.u.), high frequency (0.75-2.5 Hz) power in normalized unit; LF (n.u.), low frequency (0.2-0.75 Hz) power in normalized unit; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration.
↵ a Tested with t tests, P < 0.05 on all parameters.