Genes modulated by A2A receptor agonist CGS-21680 in HUVEC
HUVEC were incubated for 4 h in the presence or absence of 1 μM selective adenosine A2A receptor agonist, CGS-21680. The subtraction library was generated using the cDNA representational difference analysis as described under Materials and Methods. Products larger than 164 base pairs indicate that an insert had been cloned. PCR products of inserts from individual clones were identified, analyzed using Sequencher, and compared with public databases by using BLAST. Column 1 indicates the number of hits for the protein mentioned in column 2.
| Number of Clones | Homology (>99%) |
|---|---|
| 5 | Thrombospondin 1 |
| 5 | Laminin β1 |
| 2 | Catenin α |
| 1 | Catenin isoform 1A |
| 3 | Integrin-linked kinase |
| 2 | Karyopherin β1 |
| 4 | Transmembrane protein with epidermal growth factor and follistatin-like domains |
| 2 | Gap junction protein, connexin 43 |
| 1 | H-cadherin 13 |
| 1 | Human type XII collagen α1 |
| 5 | Mouse type XVIII collagen α1 |
| 2 | MUC-18, cell-surface glycoprotein |
| 2 | α Spectrin |
| 1 | Type II receptor for morphogenic proteins |
| 1 | Human C/EBP γ for interleukin-4 promoter |
| 1 | 2,3-Biphosphoglycerate |
| 1 | Similar to basonuclin |
| 1 | p40 |
| 1 | ZNF127-xp zinc finger protein |
| 1 | Mitogen-responsive phosphoprotein (DOC-2) |
| 1 | Protein phosphatase 2A, α subunit |
| 1 | Protein tyrosine phosphatase alpha |
| 2 | Ca2+ ATPase |
| 3 | Proteosome ATPase |
| 1 | ATP synthase, α subunit |
| 2 | Ras GTPase activating protein SynGAP-C |
| 1 | UMP synthase |
| 2 | UMP synthase and mitochondrial elongation factor |
| 2 | Elongation factor 2 |
| 1 | Asparaginyl-tRNA synthethase |
| 8 | Glycyl-tRNA synthethase |
| 2 | Microtubule associated protein 1B |
| 2 | Splice factor SRp30c |
| 1 | CGG trinucleotide repeat binding protein |
| 1 | Tim 17 preprotein translocase |
| 1 | Nuclear distribution protein C |
| 2 | Ribosomal protein P0 and glycyl-tRNA synthethase |
| 6 | Ribosomal protein P0 |
| 1 | Acidic ribosomal protein P0, P1, and P2 |
| 3 | Mitochondrial genome |
| 3 | EXT1 tumor suppressor gene |
| 1 | Tumor suppressor p33 ING1 homolog |
| 1 | Excision repair gene |
| 1 | Excision repair gene and KIAA0251 |
| 3 | CGI-74 or CGI-59 |
| 1 | IDN3 |
| 1 | PAC 30p20 |
| 1 | PAC 12q24 |
| 1 | CAD |
| 9 | Human clone 24901 |
| 12 | KIAA0251 |
| 1 | KIAA0676 (BUB2-like protein) |
| 1 | KIAA0333 |
| 1 | KIAA0230 (similar to peroxidasin) |
| 2 | KIAA0091 (site-1 protease) |
| 1 | KIAA1013 |
| 1 | KIAA0107 |
| 1 | KIAA0478 (zinc finger domain) |
| 1 | KIAA0225 |
| 1 | KIAA1603 |