Ligand | Total | Total t½ | Intracellular | Intracellular t½ |
---|---|---|---|---|
min −1 | min | min −1 | min | |
Control | ||||
Association | 0.0802 ± 3.865 × 10−4 | 12.47 | 0.0807 ± 3.587 × 10−4 | 12.39 |
Dissociation | 0.0824 ± 3.137 × 10−4 | 12.14 | 0.1040 ± 1.9207 × 10−4 | 9.61 |
Wash (−PTA) | ||||
Dissociation | 0.046 ± 2.51 × 10−4 | 22.00 | ||
Wash (+PTA) | ||||
Dissociation | 0.094 ± 3.41 × 10−4 | 10.70 | ||
Wash (+QAPB, +PTA) | ||||
Dissociation | 0.039 ± 2.04 × 10−4 | 25.60 |
PTA, phentolamine.