Treatment | Trypan Blue-Negative Cells | ||||
---|---|---|---|---|---|
5 min | 10 min | 15 min | 30 min | 60 min | |
% of control | |||||
Arachidonate | 97 ± 3.6 | 96 ± 3.9 | 98 ± 4.1 | 95 ± 4.8 | 97 ± 3.2 |
Cyclosporin A | 95 ± 4.6 | 98 ± 3.3 | 96 ± 2.7 | 99 ± 2.7 | 95 ± 3.5 |
AACOCF3 | 95 ± 3.8 | 94 ± 2.9 | 94 ± 4.1 | 96 ± 4.4 | 94 ± 3.8 |
N-Acetyl-l-cysteine | 99 ± 4.0 | 97 ± 2.8 | 95 ± 2.7 | 94 ± 3.3 | 93 ± 2.5 |
Catalase | 96 ± 2.9 | 95 ± 3.4 | 93 ± 3.8 | 98 ± 4.1 | 96 ± 4.7 |
Peroxynitrite (100 μM) | 94 ± 5.2 | 93 ± 6.3 | 95 ± 4.7 | 91 ± 3.8 | 91 ± 4.5 |
+ Arachidonate | 96 ± 4.5 | 97 ± 4.2 | 91 ± 5.7 | 92 ± 2.7 | 93 ± 5.1 |
+ Cyclosporin A | 97 ± 4.3 | 96 ± 3.6 | 93 ± 2.8 | 92 ± 5.8 | 93 ± 3.3 |
+ N-acetyl-l-cysteine | 95 ± 5.0 | 94 ± 3.8 | 94 ± 4.5 | 90 ± 3.9 | 92 ± 4.9 |
+ Catalase | 93 ± 5.8 | 95 ± 3.6 | 91 ± 3.3 | 94 ± 4.9 | 96 ± 4.1 |
+ AACOCF3 | 93 ± 5.5 | 89 ± 3.4 | 80 ± 4.5* | 67 ± 5.2** | 51 ± 2.9** |
+ AACOCF3 + Arachidonate | 93 ± 6.1 | 91 ± 2.4 | 91 ± 5.3 | 92 ± 4.6 | 91 ± 3.8 |
+ AACOCF3 + Cyclosporin A | 93 ± 4.7 | 92 ± 3.5 | 90 ± 3.6 | 91 ± 2.4 | 92 ± 3.9 |
+ AACOCF3 + N-Acetyl-l-cysteine | 90 ± 3.6 | 87 ± 4.8 | 81 ± 5.3* | 62 ± 3.7** | 54 ± 4.4** |
+ AACOCF3 + Catalase | 94 ± 2.9 | 83 ± 4.8 | 77 ± 3.1* | 69 ± 4.0** | 52 ± 5.1** |
Peroxynitrite (1.2 mM) | 94 ± 5.8 | 88 ± 3.6 | 81 ± 4.2* | 64 ± 5.0** | 55 ± 2.1** |
+ Arachidonate | 91 ± 4.0 | 91 ± 3.3 | 92 ± 4.5 | 89 ± 5.3 | 93 ± 6.7 |
+ Cyclosporin A | 89 ± 4.9 | 91 ± 3.0 | 90 ± 5.8 | 93 ± 6.1 | 90 ± 4.6 |
+ N-acetyl-l-cysteine | 93 ± 3.6 | 92 ± 2.5 | 94 ± 4.4 | 95 ± 5.8 | 91 ± 3.8 |
+ Catalase | 94 ± 5.3 | 89 ± 4.7 | 92 ± 4.1 | 91 ± 5.3 | 93 ± 6.1 |
↵* , P < 0.01; **, P < 0.001 compared with cells that did not receive peroxynitrite.