Yeast strains used in these studies
| Strains | Genotype |
|---|---|
| RHY718 (wt, HRD) | MATα ade2-101 met2 his3Δ200 hmg2::HIS3 lys2-801 hmg1::LYS2 leu2Δ trp1Δ ura3-52 +pRH244 (URA3, 6mycHMG2) |
| RHY609 (hrd1Δ) | MATα ade2-101 met2 his3Δ200 hmg2::HIS3 lys2-801 hmg1::LYS2 LEU trp1::hisG ura3-52::6mycHMG2 hrd1::URA3 |
| RHY925 (hrd2-1) | MATα ade2-101 met2 his3Δ200 hmg2::HIS3 lys2-801 hmg1::LYS2 leu2Δ trp1Δ URA3::6MycHMG2 hrd2-1 |
| RHY749 (hrd3Δ) | MATα ade2-101 met2 his3Δ200 hmg2::HIS3 lys2-801 hmg1::LYS2 leu2Δ trp1Δ ura3-52::6MycHMG2 hrd3::URA3 |
| RHY1166 (wt, UBC) | MATα ade2-101 met2 his3Δ200 hmg2::HIS3 lys2-801 hmg1::LYS2 leu2Δ trp1Δ ura3-52::MycHMG2 |
| RHY1596 (ubc6Δ) | MATα ade2-101 met2 his3Δ200 hmg2Δ-4 lys2-801 hmg1::LYS2 leu2Δ trp1Δ ura3-52::MycHMG2 ubc6::KanMX |
| RHY1603 (ubc7Δ) | MATα ade2-101 met2 his3Δ200 hmg2Δ-4 lys2-801 hmg1::LYS2 leu2Δ trp1Δ ura3-52::MycHMG2 ubc7::HIS3 |
| RHY1604 (ubc6Δ/7Δ) | MATα ade2-101 met2 his3Δ200 hmg2Δ-4 lys2-801 hmg1::LYS2 leu2Δ trp1Δ ura3-52::MycHMG2 ubc6::KanMX ubc7:HIS3 |
| RHY473 (wt, PEP4) | ade2-101 met2 his3Δ200 lys2-801 ura3-52 |
| RHY106-4 (pep4Δ) | ade2-101 met2 his3Δ200 lys2-801 ura3-52 pep4Δ |