TABLE 1

Mean EC₅₀ values, Hill slopes, and shifts in potency for wild-type NR1/NR2A NMDA receptors and receptors carrying mutations in the S1 and S2 ligand binding domains

For each receptor, the EC₅₀ is given on the first line and the n_H on the second. The number of oocytes studied is given in parentheses.

Agonist Construct	Glutamate	Aspartate	NMDA	Tet-Gly	Glycine
			μM
Wild type	3.5 ± 0.4	18.4 ± 1.4	47.0 ± 2.7	1.7 ± 0.2	2.6 ± 0.2
	1.2 ± 0.1 (24)	1.0 ± 0.1 (10)	1.5 ± 0.1 (5)	1.3 ± 0.2 (6)	1.5 ± 0.2 (4)
H466A	490 ± 64	1798 ± 391	3995 ± 93	71.1 ± 9.3	3.7 ± 0.2
	1.5 ± 0.2 (7)	1.3 ± 0.2 (9)	2.4 ± 0.2 (6)	1.3 ± 0.2 (10)	1.5 ± 0.1 (4)^a
H466F	46.2 ± 3.2		229 ± 21
	1.7 ± 0.2 (4)		1.4 ± 0.2 (7)
S492A	146 ± 7	4408 ± 277	1389 ± 157	35.7 ± 5.4	2.5 ± 0.3
	1.2 ± 0.1 (6)	0.9 ± 0.1 (12)	1.4 ± 0.2 (5)	1.1 ± 0.1 (6)	1.3 ± 0.2 (5)
S492P	68.0 ± 6.4		3545 ± 211
	1.4 ± 0.2 (10)		1.6 ± 0.1 (9)
T494A	482 ± 38	4488 ± 382	552 ± 78	38.5 ± 4.5	1.5 ± 0.2
	1.3 ± 0.1 (7)	0.9 ± 0.1 (12)	1.2 ± 0.2 (5)	1.4 ± 0.2 (6)	1.2 ± 0.2 (4)
S670G	421 ± 58	1999 ± 114	544 ± 19	36.1 ± 3.2	2.3 ± 0.1
	1.4 ± 0.2 (12)	1.0 ± 0.1 (12)	2.1 ± 0.2 (6)	1.6 ± 0.2 (8)	1.3 ± 0.1 (5)
T671A	2967 ± 279	1851 ± 129	351 ± 25	69.6 ± 6.1	2.9 ± 0.2
	1.3 ± 0.1 (5)^a	1.1 ± 0.1 (16)	2.2 ± 0.4 (6)	1.2 ± 0.1 (6)	1.3 ± 0.1 (4)^a
	Fold-shift in EC₅₀
H466A	140	98	85	42
H466F	13		5
S492A	42	240	30	21
S492P	19		75
T494A	138	244	12	23
S670G	120	109	12	21
T671A	848	101	7	41

↵a Values reported in Anson et al. (1998).