Oligonucleotides used in this study
| Oligonucleotide | Sequence (5′-3′) | Purpose |
|---|---|---|
| 2B6(-2253)F | TATGAATGAGAACgcGTGATATTCACT | Promoter cloning |
| 2B6(+16)R | GGACGCTGAGTTagATctTCCTGGTCTG | (Mismatches in lowercase) |
| TQ-CYP2B6FOR | GCTGAACTTGTTCTACCAGACTTTTTC | Quantitative PCR |
| TQ-CYP2B6REV | GAAAGTATTTCAAGAAGCCAGAGAAGAG | |
| CYP2B6 MGB-probe | FAM-TGTATTCGGCCAGCTGT-MGBNFQa | |
| seqCYP2B6-P1F | ACAATACCTCACTAAGAGTG | Promoter sequencing |
| seqCYP2B6-P2F | GTCTCAGTTTCTGTCTCCTTC | |
| seqCYP2B6-P3F | TGAACATGCACTACCACCA | |
| seqCYP2B6-P4R | ATCCTTGCATGTGTATGAGC | |
| seqCYP2B6-P5R | TGAACCAGGAGTAGCAAGAG | |
| 2B6-82MTF | GGGGAATGGATGAAATTTCATAACAGGGTGCAGAGGC | In vitro mutagenesis |
| 2B6-82MTR | GCCTCTGCACCCTGTTATGAAATTTCATCCATTCCCC | |
| 2B6-750MTF | ATCACGCCCGGCTAATTTTTGT | |
| 2B6-750MTR | ACAAAAATTAGCCGGGCGTGAT | |
| 2B6-801MTF | GGTTCAAGTGATTCTCTTTCCTCAGCCTCCCGAG | |
| 2B6-801MTR | CTCGGGAGGCTGAGGAAAGAGAATCACTTGAACC | |
| 2B6-1848MTF | GTAAAGCACTTCAAGCCTCCCCATCG | |
| 2B6-1848MTR | CGATGGGGAGGCTTGAAGTGCTTTAC | |
| 2B6(-3010)F | GAAAGAGACTGGCTGAATGGA | Genotyping -2320T→C |
| 2B6(-1894)R | TATTGTTGCCATCCCCATTT | |
| 2B6cDNA(41)R | CCTGTGAGGAGTGCAAGGAAGAGG | Primer extension CYP2B6 |
| 2B6cDNA(521)R | GATGGAGCAGATGATGTTGGCGGTAA | RACE primer CYP2B6 |
| luci(350)R | CACGGTAGGCTGCGAAATGCCCATA | RACE primer Luciferase |
| luci(220)R | AGCTTCTGCCAACCGAACGGACATTT | RACE nested primer Luciferase |
| 2B6EMSA1 sense/antisense | gatccTGGATGAAATTTTATAACAGGGTGCa | -94 to -70 bp EMSA (WT) |
| gatctGCACCCTGTTATAAAATTTCATCCAg | ||
| 2B6EMSA2 sense/antisense | gatccTGGATGAAATTTCATAACAGGGTGCa | -94 to -70 bp EMSA (-82C) |
| gatctGCACCCTGTTATGAAATTTCATCCAg | ||
| 2B6EMSA3 sense/antisense | gatccCAGGGTCAGGATAAAAGGCCCAGTTa | -64 to -40 bp EMSA |
| gatctAACTGGGCCTTTTATCCTGACCCTGg |
↵ a FAM, 5-carboxyfluorescein; MGB, minor groove binder; NFQ, nonfluorescent quencher.