Oligonucleotides used for ChIP assay
| PCR | 5′-NT | Primer Sequence | Product |
|---|---|---|---|
| CYP3A4 enhancer C/EBP binding site -5.95 kb (GenBank Accession no. AF185589) | -5953 | 5′-CAGGGTCAGGCAAAACAAG-3′ FP | 286 bp |
| -5667 | 5′-TTCAGCAGAAAGGCAAATGT-3′ RP | ||
| Albumin promoter C/EBP binding site (-100 bp) (GenBank Accession no. M13075) | -324 | 5′-GCAAACTCCAAGAACTAAAATGTT-3′ FP | 395 bp |
| +71 | 5′-AAAAGAAGGGAAATAAAGGTTACC-3′ RP | ||
| CYP3A4 gene Exon 2. Negative C/EBP binding site (GenBank Accession no. AF209389) | +3679 | 5′-ATCTCATCATCACTGCGGAAGA-3′ FP | 686 bp |
| +4364 | 5′-AGAGCCCTTGGGTAAACATTGC-3′ RP |
NT, nucleotide; FP, forward primer; RP, reverse primer.