Effects of gemcitabine-pemetrexed combinations on dCK and hENT1 gene expression of NSCLC cell lines
The amount of target gene, normalized to GAPDH and relative to the calibrator (untreated control cells), is given as 2-ΔΔCT.
| Effect Levels | Treatments | dCK | hENT1 |
|---|---|---|---|
| A549 Cells | |||
| Control | 1.00 | 1.00 | |
| 0.25 | Gemcitabine-pemetrexed | 1.37 | 1.38 |
| 0.25 | Pemetrexed-gemcitabine | 1.42 | 1.51 |
| 0.50 | Gemcitabine-pemetrexed | 1.45 | 2.80 |
| 0.50 | Pemetrexed-gemcitabine | 3.01 | 3.19 |
| Calu-1 Cells | |||
| Control | 1.00 | 1.00 | |
| 0.25 | Gemcitabine-pemetrexed | 1.05 | 1.42 |
| 0.25 | Pemetrexed-gemcitabine | 1.12 | 1.38 |
| 0.50 | Gemcitabine-pemetrexed | 1.37 | 1.36 |
| 0.50 | Pemetrexed-gemcitabine | 1.59 | 1.73 |
| Calu-6 Cells | |||
| Control | 1.00 | 1.00 | |
| 0.25 | Gemcitabine-pemetrexed | 0.93 | 1.54 |
| 0.25 | Pemetrexed-gemcitabine | 1.07 | 1.66 |
| 0.50 | Gemcitabine-pemetrexed | 1.87 | 1.80 |
| 0.50 | Pemetrexed-gemcitabine | 1.95 | 2.02 |