TABLE 3
Km values of wild-type and mutant Asu136 RT enzymes for poly(rC) · oligo(dG) in the presence of varying concentrations of urea
Data are the mean of three to four independent experiments. The Km values were calculated from the average Lineweaver-Burk plots shown in Fig. 7. The amounts of WT, N136T, and N136L RT enzyme used in the 50-μl assays were 0.85, 7.0, and 26 ng of protein, respectively.