Clone | Primers | Annealing Temperatures | RES | |
---|---|---|---|---|
°C | ||||
Kv3.1 I389D | 1. CTGCCCCAACAAGATAGAGTTCAT | 57 | 55 | MscI |
2. CAGAAGCCATCGGGGATGTTCTTAAAGTGGG | BstEII | |||
3. GTCATGGTGACCACGGCCCACCAGAAGCCATCGGGGA | ||||
Kv1.5 D469I | 1. AGCGGGGTCATGGCCCCGCCCTCT | 60 | 59 | PmlI |
2. ACCAGAAGGCGATAGGGATGCTAG | BstEII | |||
3. CATGGTGACCACTGCCCACCAGAAGGCGATAGGGATGC | ||||
Kv3.1 L401V | 1. ATGACGACGGTGGGCTACGGAGAC | 54 | 54 | BstEII |
2. GCCCGTGGTCACCATGACGACGGTGGGCTAC | BamHI | |||
3. ATCGGATCCTCAAGTCACTCTCAC | ||||
Kv1.5 V481L | 1. AGTGGTCACCATGACCACTCTGGGCTACGGGGA | 58 | BstEII | |
2. CATGATATCTCACAAATCTGTTTCCCGGCT | EcoRV | |||
Kv3.1 L422I | 1. GCACTGTGTGCGATAGCGGGCGTGCTG | 53 | BstAPI | |
2. ATCGGATCCTCAAGTCACTCTCAC | BamHI | |||
Kv1.5 I502L | 1. TCCAGTGCCGTCTACTTCGCA | 57 | 59 | |
2. AGGACCCCGGCGAGGGCACACAGCGAG | BstEII | |||
3. GCTGTGTGCCCTCGCCGGGGTCCTCAC | 57 | EcoRV | ||
4. CATGATATCTCACAAATCTGTTTCCCGGCT | ||||
Kv4.2 L392I | 1. GGCCCTGGTGTTCTACTATGT | 53 | 54 | |
2. GACCAAGACTCCGCTAATTGAGCAGAT | BsmBI | |||
3. GGGTCTATCTGCTCAATTAGCGGAGTC | 49 | NotI | ||
4. ATAGTTTAGCGGCCGCGTCTTACAAAGCAGACAC | ||||
Kv1.4 I547L | 1. GCAGAGGCAGATGAACCTACC | 53 | 54 | |
2. TAAGACACCCGCAAGGGCACACAG | BsmI | |||
3. GGGTCCCTGTGTGCCCTTGCGGGTGTC | 52 | BamHI | ||
4. GATGGATCCTCAGACGTCAGTCTC | ||||
Kv3.1/Kv1.5S6-Cterm | 1. CAAGATAAGCTTATGGGCCAAGGGGACGAGAGCGAG | 63 | 61 | |
2. CCCGGCGATGGCACACAGCGAGCCCACGATCATGCCGGACCACGTCTGCGGGTA | HindIII | |||
3. GACATGTACCCGCAGACGTGGTCCGGCATGATCGTGGGCTCGCTGTGTGCCATC | 63 | SacI | ||
4. ACGAATGAGCTCTCACAAATCTGTTTCCCGGCTGGT | ||||
Kv1.5/Kv3.1S6-Cterm | 1. CAAGATAAGCTTATGGAGATCGCCCTGGTGCCCCTG | 63 | 59 | |
2. GCCCGCCAGCGCACACAGTGCTCCCACCAGCTTGCCCCCAACAGTGATGGGCCT | HindIII | |||
3. GACATGAGGCCCATCACTGTTGGGGGCAAGCTGGTGGGAGCACTGTGTGCGCTG | 61 | BamHI | ||
4. ACGAATGGATCCTCAAGTCACTCTCACAGCCTCTGT | ||||
Kv3.1/Kv1.5Cterm | 1. ATAGGGGCCCAGCCCAATGAC | 59 | 49 | 54 |
2. CACGATGACGGGCACAGGCAT | ||||
3. GTTGAAGTTGGACACGATGACGGG | BstEII | |||
4. TCCAACTTCAACTACTTCTACCACCGG | 59 | 53 | SacI | |
5. ACGAATGAGCTCTCACAAATCTGTTTCCCGGCTGGT | ||||
6. CCCGTCATCGTGTCCAACTTCAACTACTTCTAC | ||||
Kv1.5/Kv3.1Cterm | 1. TCCAGTGCCGTCTACTTCGCA | 48 | 55 | |
2. GACGATGACGGGCACAGGCAG | 57 | |||
3. CCCAAAATTGTTGACGATGACGGG | BstEII | |||
4. AACAATTTTGGGATGTATTACTCC | 54 | BamHI | ||
5. ACGAATGGATCCTCAAGTCACTCTCACAGCCTCTGT | 54 | |||
6. CCCGTCATCGTCAACAATTTTGGGATGTATTACTCC |
RES, restriction endonuclease sites.