Autoradiographic analysis of GABAA-R binding sites in horizontal sections of control and conditional GABAA-R α1 subunit knockout mice
The data are means ± S.D. for five mice in each genotype and are expressed as nanocuries per milligram for 3H ligands and as nanocuries per gram for 35S ligands.
| Ligand | Brain Region | ||||
|---|---|---|---|---|---|
| Ctx | Th | Hi | CPu | Cere | |
| [3H]Ro 15-4513 binding to benzodiazepine sites | |||||
| Cre-, α1F/F mice | 121 ± 10 | 50 ± 3 | 115 ± 5 | 51 ± 4 | 97 ± 5 |
| Cre+, α1F/F mice | 90 ± 13* | 47 ± 7 | 108 ± 6 | 44 ± 6 | 103 ± 9 |
| [3H]Muscimol binding to GABA sites | |||||
| Cre-, α1F/F mice | 12.3 ± 1.4 | 17.5 ± 5.4 | 8.2 ± 1.3 | 8.8 ± 2.0 | 35.4 ± 5.6 |
| Cre+, α1F/F mice | 11.5 ± 3.1 | 15.0 ± 1.0 | 8.1 ± 1.9 | 8.3 ± 1.2 | 31.8 ± 5.3 |
| [35S]TBPS binding to ionophore sites | |||||
| Cre-, α1F/F mice | 226 ± 54 | 503 ± 94 | 157 ± 26 | 338 ± 32 | 122 ± 26 |
| Cre+, α1F/F mice | 70 ± 14** | 472 ± 128 | 121 ± 22* | 193 ± 36** | 115 ± 21 |
Significance of the difference from control (Student's t test): *P < 0.01; **P < 0.001.
Ctx, cerebral cortex; Th, thalamus; Hi, hippocampus; CPu, caudate-putamen; Cere, cerebellar cortex.