EC50 values and maximal relaxation in response to ACh and GTN of aorta from wt mice treated in vivo with GTN or sham (ethanol)
Presented are a separate set of experiments in which relaxation of aorta from wt mice treated in vivo with ethanol or GTN (low and high dose) was assessed. EC50 values were normalized on maximal relaxation.
| Group | Potency, EC50 | Efficacy | ||
|---|---|---|---|---|
| ACh | GTN | ACh | GTN | |
| -log M | % maximal relaxation | |||
| wt, in vivo ethanol | 7.10 ± 0.07 (n = 14) | 7,22 ± 0.04 (n = 12) | 71.38 ± 2.97 (n = 14) | 82.17 ± 2.38 (n = 12) |
| wt, in vivo GTN (16 μg/h) | 7.09 ± 0.13 (n = 7) | 7.03 ± 0.09 (n = 5) | 64.92 ± 3.32 (n = 7) | 84.61 ± 2.56 (n = 5) |
| wt, in vivo GTN (100 μg/h) | 6.95 ± 0.11 (n = 11) | 6.43 ± 0.15**† (n = 9) | 54.59 ± 3.97** (n = 11) | 74.96 ± 3.07*†† (n = 9) |
*Significance (P < 0.01) versus wt, in vivo ethanol group (** means P < 0.005).
†Significance (P < 0.05) versus wt, in vivo GTN (16 μg/h) group (†† means P < 0.01).