Variable Substrate or Template/Primer | Reverse Transcriptase | |
---|---|---|
Wild-Type | T139I | |
dGTP | ||
K m | 0.50 ± 0.05 μM | 0.8 ± 0.2 μM |
k cat | 0.015 ± 0.004 s−1 | 0.014 ± 0.001 s−1 |
kcat/Km | 0.03 μM−1 s−1 | 0.018 μM−1 s−1 |
Poly(rC)·oligo(dG)12-18 | ||
K m | 0.25 ± 0.08 μM | 0.5 ± 0.2 μM |
k cat | 0.15 ± 0.01 s−1 | 0.17 ± 0.04 s−1 |
kcat/Km | 0.6 μM−1 s−1 | 0.34 μM−1 s−1 |