Primers used for PCR reactions
Mutant nucleotides are underlined.
| Primers | Sequence |
|---|---|
| Flanking | |
| Forward | CCACAATGTCACTGAGAAGGTCACCCAGG |
| Reverse | GCAGTGAGCGGTTCAGGTGCAGGCAGATGTC |
| S620T | |
| Forward | CTACTTCACCTTCACCAGCCTCACCAGTGTGGG |
| Reverse | CCCACACTGGTGAGGCTGGTGAAGGTGAAGTAG |
| F656V | |
| Forward | ATGTATGCTAGCATCGTCGGCAACGTGTCGGC |
| Reverse | GCCGACACGTTGCCGACGATGCTAGCATACAT |
| F656M | |
| Forward | ATGTATGCTAGCATCATGGGCAACGTGTCGGC |
| Reverse | GCCGACACGTTGCCCATGATGCTAGCATACAT |