Expression levels and [3H]diprenorphine binding affinity of hDOP-Gi1αC351I fusion proteins
Data represent means ± S.E.M. of n = 4 experiments performed on different membrane preparations.
| Construct | Bmax | pKd |
|---|---|---|
| fmol/mg of protein | ||
| hDOP-Gi1αC351I | 1816 ± 209 | 9.20 ± 0.03 |
| hDOPV150E, V154D-Gi1αC351I | 2181 ± 228 | 8.78 ± 0.01*** |
| hDOP-Gi1αG202A,C351I | 1777 ± 285 | 9.19 ± 0.05 |
| hDOPV150E, V154D-Gi1αC351I + hDOP-Gi1αG202A, C351I | 2310 ± 301 | 8.85 ± 0.02*** |
↵*** Significantly different from hDOP-Gi1αC351I, P < 0.001.