TABLE 2

Binding affinity of DADLE for individually expressed and co-expressed hDOP-G i1αC351I fusion proteins

Data represent means ± S.E.M. of n = 4 experiments performed in triplicate on different membrane preparations.

Construct pKhKh Sites pK1 Hill Number
%
hDOP-Gi1αC351I 9.03 ± 0.18 63 ± 6 6.79 ± 0.42 −0.39 ± 0.03
hDOPV150E, V154D-Gi1αC351I 7.40 ± 0.24** 57 ± 8 4.99 ± 0.37* −0.50 ± 0.02
hDOP-Gi1αG202A, C351I 8.70 ± 0.12 59 ± 3 5.82 ± 0.23 −0.34 ± 0.04
hDOPV150E, V154D-Gi1αC351I + hDOP-Gi1αG202AC351I 8.69 ± 0.15 45 ± 9 6.29 ± 0.28 −0.41 ± 0.04

  • ↵* Significantly different from hDOP-Gi1αC351I, P < 0.05.

  • ↵** Significantly different from hDOP-Gi1αC351I, P < 0.01.