TABLE 3

Binding affinity of DPDPE for individually expressed and co-expressed hDOP-G i1αC351I fusion proteins

Data represent means ± S.E.M. of n = 4 experiments performed in triplicate on different membrane preparations.

Construct pKhKh Sites pK1 Hill Number
%
hDOP-Gi1αC351I 8.79 ± 0.09 70 ± 3 5.89 ± 0.25 −0.41 ± 0.005
hDOPV150E, V154D-Gi1αC351I 7.42 ± 0.11** 51 ± 20 5.48 ± 0.41 −0.61 ± 0.04
hDOP-Gi1αG202A, C351I 8.88 ± 0.15 65 ± 4 5.82 ± 0.38 −0.40 ± 0.03
hDOPV150E, V154D- Gi1αC351I + hDOP-Gi1αG202A, C351I 8.31 ± 0.28 55 ± 12 6.10 ± 0.35 −0.46 ± 0.01

  • ↵** Significantly different from hDOR-Gi1αC351I, P < 0.01, one-way ANOVA.