Expression levels and [3H]diprenorphine binding affinity of hMOP-Gi1αC351I fusion proteins
Data represent means ± S.E.M. from n = 3 experiments performed in triplicate on different membrane preparations. Statistics were performed using one-way ANOVA on Bmax and pKd numbers.
| Construct | Bmax | pKd |
|---|---|---|
| fmol/mg | ||
| hMOP-Gi1αC351I | 1217 ± 72 | 9.47 ± 0.08 |
| hMOPV169E, V173D-Gi1αC351I | 901 ± 110 | 9.39 ± 0.17 |
| hMOP-Gi1αG202A, C351I | 1251 ± 20 | 9.52 ± 0.08 |
| hMOPV169E, V173D-Gi1αC351I + hMOP-Gi1αG202A, C351I | 1285 ± 120 | 9.56 ± 0.10 |