Expression of nAChR subunits in the superior colliculus of α4, α6, and β3 +/+ and -/mice The reported values are expressed as femtomoles of specific labeled [3H]Epi binding sites per milligram of protein and are the mean values ± S.E.M. of three to four experiments performed in triplicate per group, or as percentage of each subunit Ko mean value versus the respective WT mean value.
| nAChR subunit | α4+/+ | α4–/– | % | α6+/+ | α6–/– | % | β3+/+ | β3–/– | % |
|---|---|---|---|---|---|---|---|---|---|
| α2 | 3.4 ± 1.0 | 2.2 ± 1.5 | 64.7 | 4.3 ± 2.5 | 5.4 ± 3.9 | 124.9 | 7.5 ± 3.9 | 5.1 ± 3.3 | 68.0 |
| α3 | 25.7 ± 6.8 | 22.8 ± 3.9 | 88.7 | 24.7 ± 5.4 | 17.8 ± 3.0 | 72.1 | 28.5 ± 3.5 | 23.9 ± 7.5 | 83.9 |
| α4 | 160.4 ± 3.7 | 2.5 ± 1.9*** | 1.6 | 146.9 ± 10.6 | 147.1 ± 8.2 | 100.1 | 150.0 ± 13.3 | 132.1 ± 22.0 | 88.1 |
| α5 | 10.5 ± 2.4 | 3.6 ± 0.3* | 34.2 | 9.3 ± 3.2 | 9.2 ± 4.7 | 98.9 | 9.4 ± 2.3 | 8.2 ± 3.1 | 87.2 |
| α6 | 80.3 ± 9.7 | 38.3 ± 6.5* | 47.7 | 88.3 ± 4.4 | 1.3 ± 0.4*** | 1.5 | 60.6 ± 7.5 | 15.8 ± 1.5*** | 26.1 |
| β2 | 216.7 ± 15.9 | 56.3 ± 7.8*** | 26.0 | 242.4 ± 9.7 | 167.5 ± 10.8** | 69.1 | 219.1 ± 17.3 | 155.2 ± 16.2* | 70.8 |
| β3 | 70.6 ± 2.2 | 26.9 ± 2.2*** | 38.1 | 72.0 ± 5.7 | 21.6 ± 2.3*** | 30.0 | 65.0 ± 3.5 | 1.6 ± 0.7*** | 2.5 |
| β4 | 7.0 ± 2.2 | 4.3 ± 1.2 | 61.4 | 3.9 ± 1.6 | 3.1 ± 2.0 | 79.5 | 4.7 ± 2.0 | 0.3 ± 0.2 | 7.0 |
Statistical analysis according to unpaired Student t test: * P < 0.05; ** P < 0.01; *** P < 0.001 Ko versus respective WT values.