TABLE 1

pTracer-CMV2 (Invitrogen, Carlsbad, CA) clones The parent plasmid contains a CMV promoter that yields robust expression of cloned sequences when transfected into mammalian cells. This plasmid is referred to in the text as “CMV2”.

Name PCR Primers Amino Acids Restriction Sites
CAR1 FP: GATCGAATTCGTCATGGCCAGTAGGGAAGATGAG 1–348 EcoR1/EcoRV
RP: GATCGATATCTCAGCTGCAGATCTCCTGGAGCCAG
CAR3 FP: GATCGAATTCGTCATGGCCAGTAGGGAAGATGAG 1–353 EcoR1/EcoRV
RP: GATCGATATCTCAGCTGCAGATCTCCTGGAGCCAG
  • FP, forward primer; RP, reverse primer.