Genes whose deletion confers hypersensitivity to TPZ Genes whose deletion results in hypersensitivity to TPZ are listed along with their systematic names (ORF) and their cellular function (if known). See Fig. 3 for examples of relative sensitivities.
| Gene | ORF | Score | Cellular Function and Comment |
|---|---|---|---|
| DNA repair and genome stability | |||
| ASF1 | YJL115W | – – – | Target of the Rad53-dependent DNA damage response |
| MMS1 | YPR164W | – – – | Required for repair of replication-dependent DNA damage |
| MMS4 | YBR098W | – – – | Required with Mus81 for repair of DNA damage by MMS |
| MMS22 | YLR320W | – – – | Acts in a DNA repair pathway with Mms1 |
| MRE11 | YMR224C | – – – | Single-stranded endonuclease and double-stranded exonuclease required for double-strand break repair |
| MUS81 | YDR386W | – – – | Part of a complex with Rad54 and Mms4 |
| NCE4 | YPL024W | – – | Synthetic interaction pattern suggests a role in DNA repair |
| RAD5 | YLR032W | – – – | Single-stranded DNA-dependent ATPase involved in error-free DNA repair |
| RAD10 | YML095C | – – – | Component of the nucleotide excision repairosome |
| RAD16 | YBR114W | – – | DNA helicase, subunit of the nucleotide excision repair factor NEF4 |
| RAD50 | YNL250W | – – – | Coiled-coil protein required for resection at double-stranded breaks and for DNA repair |
| RAD51 | YER095W | – – – | Stimulates pairing and strand-exchange between homologous single-stranded and double-stranded DNA |
| RAD52 | YML032C | – – – | Required for recombination and repair of X-ray damage |
| RAD54 | YGL163C | – – – | DNA dependent ATPase required for X-ray damage repair |
| RAD55 | YDR076W | – – – | With Rad57 promotes DNA strand exchange by Rad51 recombinase |
| RAD57 | YDR004W | – – – | With Rad55 promotes DNA strand exchange by Rad51 recombinase |
| RAD59 | YDL059C | – – – | Homolog of Rad52 involved in homologous recombination and DNA repair |
| RTT101 | YJL047C | – – – | Ubiquitin protein ligase possibly involved in genomic stability |
| RTT107 | YHR154W | – – | Functions in DNA synthesis after DNA damage during S-phase |
| RTT109 | YLL002W | – – – | Involved in resistance to mutagens such as diepoxybutane and mitomycin C |
| TOP3 | YLR234W | – – – | DNA topoisomerase III |
| UBC13 | YDR092W | – – – | Ubiquitin-conjugating (E2) enzyme involved in Rad6-dependent postreplicative repair |
| WSS1 | YHR134W | – – | Involved in sensitivity to UV irradiation |
| XRS2 | YDR369C | – – – | Required for DNA repair and meiotic recombination |
| YBR094W | – – – | Synthetic interaction pattern suggests a role in DNA repair | |
| Reductases and related proteins | |||
| PRO2 | YOR323C | – – – | γ-Glutamyl phosphate reductase (phosphoglutamate dehydrogenase) |
| Cell stress and signal transduction | |||
| BCK1 | YJL095W | – – – | Bypass requirement for protein kinase C homolog; mitogen-activated protein kinase kinase kinase |
| LYS7 | YMR038C | – – – | Copper chaperone for superoxide dismutase Sod1 |
| SIT4 | YDL047W | – – – | Protein phosphatase of the PP2A family |
| SLT2 | YHR030C | – – – | Protein kinase of MAP kinase family |
| SOD1 | YJR104C | – – – | Copper, zinc superoxide dismutase |
| SOD2 | YHR008C | – – | Manganese superoxide dismutase, mitochondrial |
| Lipid, fatty acid, and sterol metabolism | |||
| ERG2 | YMR202W | – – – | C-8 sterol isomerase, ergosterol biosynthesis enzyme |
| ERG3 | YLR056W | – – | C-5 sterol desaturase, ergosterol biosynthesis enzyme |
| ERG4 | YGL012W | – – | C-4(28) sterol reductase, ergosterol biosynthesis enzyme |
| Vesicular transport | |||
| RIC1 | YLR039C | – – – | In complex with Rgp1 to form as a guanyl-nucleotide exchange factor for Ypt6 |
| SNF7 | YLR025W | – – – | ESCRT-III subunit, functions in protein sorting to the prevacuolar endosome |
| SNF8 | YPL002C | – – | ESCRT-II subunit, functions in protein sorting to the prevacuolar endosome |
| VAM3 | YOR106W | – – – | Syntaxin homolog (t-SNARE), required for vacuolar assembly |
| VPS25 | YJR102C | – – | ESCRT-II subunit, functions in protein sorting to the prevacuolar endosome |
| VPS28 | YPL065W | – – | Required for traffic to the vacuole through the endocytic and biosynthetic pathways |
| VPS41 | YDR080W | – – – | Required for formation of adaptor protein (AP)-3 transport vesicles |
| WHI6 | YKR020W | – – – | Class B vacuolar sorting protein |
| Vacuole | |||
| PPA1 | YHR026W | – – | Component of the V0 subcomplex of the vacuolar H+-ATPase |
| TFP3 | YPL234C | – – – | Component of the V0 subcomplex of the vacuolar H+-ATPase |
| VMA4 | YOR332W | – – – | Component of the V1 subcomplex of the vacuolar H+-ATPase |
| VMA7 | YGR020C | – – – | Component of the V0 subcomplex of the vacuolar H+-ATPase |
| VMA10 | YHR039C-A | – – – | Component of the V1 subcomplex of the vacuolar H+-ATPase |
| Protein synthesis and degradation | |||
| RPS4A | YJR145C | – – – | Ribosomal protein S4A |
| ZUO1 | YGR285C | – – – | Zuotin associates with Ssz1 to form the ribosome-associated complex |
| Transcription, RNA processing | |||
| DBF2 | YGR092W | – – | Serine/threonine protein kinase of the CCR4-NOT transcriptional complex |
| GAL11 | YOL051W | – – – | Component of RNA polymerase II holoenzyme and Kornberg's mediator complex |
| PGD1 | YGL025C | – – – | Component of RNA polymerase II holoenzyme and mediator subcomplex |
| POP2 | YNR052C | – – – | Component of the CCR4 complex |
| ROX3 | YBL093C | – – – | Component of RNA polymerase II holoenzyme and mediator subcomplex |
| RPB9 | YGL070C | – – – | Nonessential subunit of RNA polymerase II |
| RSC2 | YLR357W | – – | Component of the abundant RSC complex involved in chromatin remodeling |
| SPT10 | YJL127C | – – – | Amplifies the magnitude of transcriptional regulation at various loci |
| SPT20 | YOL148C | – – – | Component of the histone acetyltransferase SAGA complex |
| SRB2 | YHR041C | – – | Component of RNA polymerase II holoenzyme and Kornberg's mediator complex |
| SW14 | YER111C | – – – | Transcription factor involved in cell cycle-dependent gene expression |
| UAF30 | YOR295W | – – | Upstream activation factor complex component; synthetic lethal with top1 mutation |
| Other functions | |||
| AKR1 | YDR264C | – – – | Ankyrin repeat-containing protein, inhibitor of signaling in the pheromone pathway |
| ALF1 | YNL148C | – – – | α-Tubulin folding cofactor B, assists in formation of the α-β-tubulin heterodimer |
| BEM1 | YBR200W | – – – | SH3-domain protein maintaining Cdc42-Cdc24 at the bud tip |
| BEM4 | YPL161C | – – – | Bud emergence protein that activates Cdc42 |
| BUD20 | YLR074C | – – | Putative nuclear pore protein |
| CIK1 | YMR198W | – – – | Spindle pole body associated protein |
| MDM20 | YOL076W | – – – | Required for N-terminal acetylation of Tpm1 necessary for actin cable organization |
| MOG1 | YJR074W | – – | Involved in nuclear protein import, interacts with Gsp1 |
| NUP188 | YML103C | – – – | Nucleoporin |
| SLA1 | YBL007C | – – – | Cytoskeleton assembly control protein |
| YNL171C | – – – | Overlaps with 3′ of the essential gene APC1/YNL172W | |
Scores for strain sensitivities are given: – – –, hypersensitive strain; – –, sensitive strain. SNARE, soluble N-ethylmaleimide-sensitive factor attachment protein receptor.