Gene | ORF | Score | Cellular Function and Comment |
---|---|---|---|
DNA repair and genome stability | |||
ASF1 | YJL115W | – – – | Target of the Rad53-dependent DNA damage response |
MMS1 | YPR164W | – – – | Required for repair of replication-dependent DNA damage |
MMS4 | YBR098W | – – – | Required with Mus81 for repair of DNA damage by MMS |
MMS22 | YLR320W | – – – | Acts in a DNA repair pathway with Mms1 |
MRE11 | YMR224C | – – – | Single-stranded endonuclease and double-stranded exonuclease required for double-strand break repair |
MUS81 | YDR386W | – – – | Part of a complex with Rad54 and Mms4 |
NCE4 | YPL024W | – – | Synthetic interaction pattern suggests a role in DNA repair |
RAD5 | YLR032W | – – – | Single-stranded DNA-dependent ATPase involved in error-free DNA repair |
RAD10 | YML095C | – – – | Component of the nucleotide excision repairosome |
RAD16 | YBR114W | – – | DNA helicase, subunit of the nucleotide excision repair factor NEF4 |
RAD50 | YNL250W | – – – | Coiled-coil protein required for resection at double-stranded breaks and for DNA repair |
RAD51 | YER095W | – – – | Stimulates pairing and strand-exchange between homologous single-stranded and double-stranded DNA |
RAD52 | YML032C | – – – | Required for recombination and repair of X-ray damage |
RAD54 | YGL163C | – – – | DNA dependent ATPase required for X-ray damage repair |
RAD55 | YDR076W | – – – | With Rad57 promotes DNA strand exchange by Rad51 recombinase |
RAD57 | YDR004W | – – – | With Rad55 promotes DNA strand exchange by Rad51 recombinase |
RAD59 | YDL059C | – – – | Homolog of Rad52 involved in homologous recombination and DNA repair |
RTT101 | YJL047C | – – – | Ubiquitin protein ligase possibly involved in genomic stability |
RTT107 | YHR154W | – – | Functions in DNA synthesis after DNA damage during S-phase |
RTT109 | YLL002W | – – – | Involved in resistance to mutagens such as diepoxybutane and mitomycin C |
TOP3 | YLR234W | – – – | DNA topoisomerase III |
UBC13 | YDR092W | – – – | Ubiquitin-conjugating (E2) enzyme involved in Rad6-dependent postreplicative repair |
WSS1 | YHR134W | – – | Involved in sensitivity to UV irradiation |
XRS2 | YDR369C | – – – | Required for DNA repair and meiotic recombination |
YBR094W | – – – | Synthetic interaction pattern suggests a role in DNA repair | |
Reductases and related proteins | |||
PRO2 | YOR323C | – – – | γ-Glutamyl phosphate reductase (phosphoglutamate dehydrogenase) |
Cell stress and signal transduction | |||
BCK1 | YJL095W | – – – | Bypass requirement for protein kinase C homolog; mitogen-activated protein kinase kinase kinase |
LYS7 | YMR038C | – – – | Copper chaperone for superoxide dismutase Sod1 |
SIT4 | YDL047W | – – – | Protein phosphatase of the PP2A family |
SLT2 | YHR030C | – – – | Protein kinase of MAP kinase family |
SOD1 | YJR104C | – – – | Copper, zinc superoxide dismutase |
SOD2 | YHR008C | – – | Manganese superoxide dismutase, mitochondrial |
Lipid, fatty acid, and sterol metabolism | |||
ERG2 | YMR202W | – – – | C-8 sterol isomerase, ergosterol biosynthesis enzyme |
ERG3 | YLR056W | – – | C-5 sterol desaturase, ergosterol biosynthesis enzyme |
ERG4 | YGL012W | – – | C-4(28) sterol reductase, ergosterol biosynthesis enzyme |
Vesicular transport | |||
RIC1 | YLR039C | – – – | In complex with Rgp1 to form as a guanyl-nucleotide exchange factor for Ypt6 |
SNF7 | YLR025W | – – – | ESCRT-III subunit, functions in protein sorting to the prevacuolar endosome |
SNF8 | YPL002C | – – | ESCRT-II subunit, functions in protein sorting to the prevacuolar endosome |
VAM3 | YOR106W | – – – | Syntaxin homolog (t-SNARE), required for vacuolar assembly |
VPS25 | YJR102C | – – | ESCRT-II subunit, functions in protein sorting to the prevacuolar endosome |
VPS28 | YPL065W | – – | Required for traffic to the vacuole through the endocytic and biosynthetic pathways |
VPS41 | YDR080W | – – – | Required for formation of adaptor protein (AP)-3 transport vesicles |
WHI6 | YKR020W | – – – | Class B vacuolar sorting protein |
Vacuole | |||
PPA1 | YHR026W | – – | Component of the V0 subcomplex of the vacuolar H+-ATPase |
TFP3 | YPL234C | – – – | Component of the V0 subcomplex of the vacuolar H+-ATPase |
VMA4 | YOR332W | – – – | Component of the V1 subcomplex of the vacuolar H+-ATPase |
VMA7 | YGR020C | – – – | Component of the V0 subcomplex of the vacuolar H+-ATPase |
VMA10 | YHR039C-A | – – – | Component of the V1 subcomplex of the vacuolar H+-ATPase |
Protein synthesis and degradation | |||
RPS4A | YJR145C | – – – | Ribosomal protein S4A |
ZUO1 | YGR285C | – – – | Zuotin associates with Ssz1 to form the ribosome-associated complex |
Transcription, RNA processing | |||
DBF2 | YGR092W | – – | Serine/threonine protein kinase of the CCR4-NOT transcriptional complex |
GAL11 | YOL051W | – – – | Component of RNA polymerase II holoenzyme and Kornberg's mediator complex |
PGD1 | YGL025C | – – – | Component of RNA polymerase II holoenzyme and mediator subcomplex |
POP2 | YNR052C | – – – | Component of the CCR4 complex |
ROX3 | YBL093C | – – – | Component of RNA polymerase II holoenzyme and mediator subcomplex |
RPB9 | YGL070C | – – – | Nonessential subunit of RNA polymerase II |
RSC2 | YLR357W | – – | Component of the abundant RSC complex involved in chromatin remodeling |
SPT10 | YJL127C | – – – | Amplifies the magnitude of transcriptional regulation at various loci |
SPT20 | YOL148C | – – – | Component of the histone acetyltransferase SAGA complex |
SRB2 | YHR041C | – – | Component of RNA polymerase II holoenzyme and Kornberg's mediator complex |
SW14 | YER111C | – – – | Transcription factor involved in cell cycle-dependent gene expression |
UAF30 | YOR295W | – – | Upstream activation factor complex component; synthetic lethal with top1 mutation |
Other functions | |||
AKR1 | YDR264C | – – – | Ankyrin repeat-containing protein, inhibitor of signaling in the pheromone pathway |
ALF1 | YNL148C | – – – | α-Tubulin folding cofactor B, assists in formation of the α-β-tubulin heterodimer |
BEM1 | YBR200W | – – – | SH3-domain protein maintaining Cdc42-Cdc24 at the bud tip |
BEM4 | YPL161C | – – – | Bud emergence protein that activates Cdc42 |
BUD20 | YLR074C | – – | Putative nuclear pore protein |
CIK1 | YMR198W | – – – | Spindle pole body associated protein |
MDM20 | YOL076W | – – – | Required for N-terminal acetylation of Tpm1 necessary for actin cable organization |
MOG1 | YJR074W | – – | Involved in nuclear protein import, interacts with Gsp1 |
NUP188 | YML103C | – – – | Nucleoporin |
SLA1 | YBL007C | – – – | Cytoskeleton assembly control protein |
YNL171C | – – – | Overlaps with 3′ of the essential gene APC1/YNL172W |
Scores for strain sensitivities are given: – – –, hypersensitive strain; – –, sensitive strain. SNARE, soluble N-ethylmaleimide-sensitive factor attachment protein receptor.