Treatment and Genotype | SNpc Counts (CE, CV) | Changea | Changeb | TH-ir Neuronsb | SNpcc | Density of DAT-ir Terminals (CE, CV) | Changea | Changeb | TT | Changea | Changeb |
---|---|---|---|---|---|---|---|---|---|---|---|
% | % | % | % | % | % | % | |||||
WT (n = 14) | 6001 ± 251 (0.077,0.042) | 0.0 | 0.0 | 5412 ± 255 (0.068, 0.047) | 90.4 | 12.3 ± 0.8 × 10–3 (0.045, 0.065) | 0.0 | 0.0 | 20.5 ± 1.3 × 10–7 | 0.0 | 0.0 |
α4(–/–) (n = 14) | 5684 ± 377 (0.073, 0.066) | 5.3 | 0.0 | 5024 ± 450 (0.082, 0.057) | 88.4 | 14.4 ± 1.5 × 10–3 (0.052, 0.095) | 17.3* | 0.0 | 25.4 ± 1.3 × 10–7 | 23.8* | –0.06 |
WT Saline (n = 12) | 5984 ± 402 (0.055, 0.079) | –1.8 | –1.7 | 12.1 ± 1.3 × 10–3 (0.041, 0.092) | –1.6 | –1.6 | 20.2 ± 1.7 × 10–7 | 0.0 | 0.0 | ||
α4(–/–) Saline (n = 12) | 5799 ± 342 (0.068, 0.079) | 3.4 | 1.2 | 14.7 ± 1.7 × 10–3 (0.042, 0.098) | 19.2* | 2.1 | 24.9 ± 2.9 × 10–7 | 21.4* | –1.6 | ||
WT Haloperidol (n = 11) | 5940 ± 519 (0.083, 0.087) | –1.0 | –1.0 | 16.5 ± 1.2 × 10–3 (0.052, 0.07) | 34.2* | 34.2† | 27.8 ± 2.0 × 10–7 | 35.6* | 35.6† | ||
α4(–/–) Haloperidol (n = 11) | 5974 ± 402 (0.073, 0.067) | 0.4 | 3.1 | 14.3 ± 1.6 × 10–3 (0.046, 0.067) | 15.8* | –0.7 | 23.9 ± 2.2 × 10–7 | 16.6* | –1.4 | ||
WT Cocaine (n = 12) | 5987 ± 170 (0.059, 0.028) | 0.2 | –0.2 | 9.9 ± 0.5 × 10–3 (0.049, 0.046) | –19.4* | –19.4† | 16.2 ± 2.9 × 10–7 | –20.8* | –20.8† | ||
α4(–/–) Cocaine (n = 12) | 5649 ± 379 (0.049, 0.083) | 5.9 | –1.3 | 14.4 ± 1.1 × 10–3 (0.050, 0.076) | 17.3* | 0.0 | 25.5 ± 1.9 × 10–7 | 24.6* | 0.5 | ||
WT Amphetamine (n = 10) | 6041 ± 524 (0.044, 0.087) | 0.7 | 0.0 | 11.2 ± 1.4 × 10–3 (0.088, 0.104) | –9.3* | –9.3† | 18.5 ± 2.3 × 10–7 | –9.9* | –9.9† | ||
α4(–/–) Amphetamine (n = 10) | 5975 ± 459 (0.062, 0.077) | 0.4 | 3.7 | 14.5 ± 1.5 × 10–3 (0.088, 0.104) | 18.1* | 0.7 | 24.3 ± 2.5 × 10–7 | 18.6* | –4.2 | ||
WT Nicotine (n = 12) | 5775 ± 434 (0.064, 0.077) | –3.7 | –3.7 | 11.7 ± 1.1 × 10–3 (0.060, 0.095) | –4.9 | –4.9 | 20.7 ± 1.6 × 10–7 | 1.1 | 1.1 | ||
α4(–/–) Nicotine (n = 10) | 5913 ± 198 (0.064, 0.033) | –1.5 | 4.0 | 14.2 ± 1.1 × 10–3 (0.049, 0.079) | 15.6* | –1.4 | 24.1 ± 1.9 × 10–7 | 17.5* | –5.1 |
n, number of hemispheres; TT, terminal tree size (see Results: this number is representative of the size of the terminal arbor and is useful for comparison rather than an accurate reflection of absolute size; TT, DAT-ir terminal density/number of SNpc neurons).
↵* , significantly different from untreated WT
↵† , significantly different from untreated group of the same genotype
↵ a Percentage of change from untreated WT
↵ b Percentage of change from untreated animals of same genotype
↵ c Percentage of TH-ir neurons in the SNpc