Potency and relative efficacy of agonists at wild-type NR1/NR2B and mutant NR1/NR2B(S690A) Parameters measured from steady-state currents by two-electrode voltage-clamp recordings on X. laevis oocytes. Mean EC50 ± S.E.M., mean respective Hill coefficients (nH), and mean relative Imax ± S.E.M. are calculated as in Tables 1 and 2. The number in parentheses (N) denotes the number of oocytes.
| Agonist | NR1/NR2B | NR1/NR2B(S690A) | ||||
|---|---|---|---|---|---|---|
| EC50 (N) | nH | Relative Imax (N) | EC50 (N) | nH | Relative Imax (N) | |
| μM | μM | |||||
| Glutamate | 1.6 ± 0.1 (4) | 1.7 | 0.6 ± 0.1 (6) | 1.3 | ||
| CCG | 0.055 ± 0.002 (9) | 1.7 | 1.08 ± 0.02 (9) | 0.034 ± 0.001 (5) | 1.9 | 1.18 ± 0.01 (5) |
| HQ | 21 ± 2 (9) | 1.5 | 1.04 ± 0.02 (9) | 2.0 ± 0.1 (6) | 1.3 | 1.28 ± 0.01 (6) |
| NMDA | 22 ± 2 (7) | 1.4 | 0.77 ± 0.01 (4) | 2.5 ± 0.2 (4) | 1.5 | 0.93 ± 0.02 (4) |
| NHP5G | 14 ± 1 (4) | 1.4 | 0.61 ± 0.02 (4) | 21 ± 1 (5) | 1.8 | 0.78 ± 0.02 (5) |
| Cl-NHP5G | 59 ± 4 (6) | 1.2 | 0.33 ± 0.01 (6) | 72 ± 10 (4) | 1.5 | 0.67 ± 0.05 (4) |
| AMAA | 47 ± 6 (6) | 1.5 | 0.58 ± 0.02 (32) | 19 ± 1 (5) | 1.2 | 1.15 ± 0.03 (28) |