HPLC analysis of APAP and its metabolites 2 h after APAP treatment Data are mean ± S.D. (n = 5).
| Liver | Serum | |
|---|---|---|
| nmol/g | nmol/ml | |
| APAP | ||
| WT | 932 ± 263 | 68 ± 22 |
| KO | 1496 ± 559 | 130 ± 41 |
| APAP-GSH | ||
| WT | 217 ± 53 | 21 ± 7 |
| KO | 3194 ± 666** | 28 ± 6 |
| APAP-CG/CYS | ||
| WT | 6 ± 3 | N.D. |
| KO | 11 ± 4 | N.D. |
| APAP-NAC | ||
| WT | N.D. | N.D. |
| KO | N.D. | N.D. |
| APAP-SUL | ||
| WT | N.D. | N.D. |
| KO | N.D. | N.D. |
| APAP-GLU | ||
| TW | N.D. | N.D. |
| KO | N.D. | N.D. |
WT, wild type; KO, knockout; N.D., not detectable.
↵** P < 0.01