Binding | |
---|---|
cpm/cell (n) | |
Rat α7 wild-type | |
20 nM MLA alone | 143 ± 4 (5)* |
20 nM MLA + 5 mM nicotine | 100 ± 12 (4) |
Rat α7 T6′S mutant | |
20 nM MLA alone | 169 ± 10 (5)* |
20 nM MLA + 5 mM nicotine | 106 ± 6 (5) |
Uninjected oocytes | |
20 nM MLA alone | 98 ± 17 (4) |
MLA, methyllycaconitine.
↵* P < 0.05 by Student's t test compared with the same receptor subtype in the presence of 20 nM methyllycaconitine with 5 mM nicotine