TABLE 3

Parameters of tetrodotoxin-dependent binding of [³H]BW202W92 to rat forebrain synaptosomes under normal and depolarizing conditions Data are means ± S.E.M.

	Method	K_D	B_max	n
		nM	fmol / mg of protein
1 mM K⁺	Saturation	21 ± 2	832 ± 129	3
1 mM K⁺	Scatchard	21 ± 2	823 ± 108	3
100 mM K⁺	Saturation	96 ± 18^a	648 ± 108	4
100 mM K⁺	Scatchard	107 ± 7^a	660 ± 54	4

↵ a P < 0.05 versus value at 1 mM K⁺. The concentration of tetrodotoxin was 1 μM in all cases