Cytotoxicity and S-glutathionylation properties of PABA/NO and related compounds
HL60 cells were treated with varying concentrations of PABA/NO and its metabolites. Cell viability was measured after 72 h with the use of the MTT assay. The IC50 mean value of three independent experiments ± S.D. is shown. Immunoblot analyses of lysates treated with 0 to 250 μM PABA/NO or its metabolites for 2 h show that S-glutathionylation is specific to intact PABA/NO.