No TCDD Pretreatment | TCDD Pretreatment | Benzo[a]pyrene 125 mg/kg/day × 5 daysa | |||||
---|---|---|---|---|---|---|---|
AUC | Clearance | Half-Life | AUC | Clearance | Half-Life | ||
min × g/ml | ml/min/kg | min | min × g/ml | ml/min/kg | min | ng/ml | |
Cyp1 +/+ | 32 ± 9.1 | 490 ± 134 | 31 ± 11 | 16 ± 5.0 | 984 ± 310 | 16 ± 3.2 | 2.2 ± 1.6 |
Cyp1a1 –/– | 121 ± 8 | 123 ± 10 | 61 ± 13 | 119 ± 34 | 135 ± 40 | 63 ± 10 | 56 ± 5.7 |
Cyp1a2 –/– | 37 ± 11 | 418 ± 94 | 36 ± 6.2 | 26 ± 4.9 | 629 ± 59 | 18 ± 3.4 | 1.5 ± 0.51 |
Cyp1b1 –/– | 53 ± 13 | 327 ± 92 | 46 ± 2.9 | 11 ± 4.5 | 979 ± 280 | 14 ± 4.1 | 5.6 ± 9.1 |
Cyp1a1/1b1 –/– | 140 ± 78 | 106 ± 75 | 70 ± 41 | 151 ± 80 | 115 ± 50 | 77 ± 49 | 165 ± 46 |
Cyp1a2/1b1 –/– | 35 ± 5.2 | 452 ± 76 | 48 ± 12 | 17 ± 7.2 | 999 ± 270 | 13 ± 7.1 | 4.0 ± 3.3 |
↵ a Whole-blood benzo[a]pyrene concentrations, after 5 days of benzo[a]pyrene feeding at the 125 mg/kg/day dose. Individual mice (n = 3) were tested between 10 and 11 AM each morning on days 3, 4, and 5; these values were similar on all 3 days and are averaged together, thus confirming that by days 3 to 5, the benzo[a]pyrene had already reached steady-state levels in the blood