[Ponasterone] | Maximal Superactivation Level | ||
---|---|---|---|
Morphine | Etorphine | Methadone | |
% | |||
0.2 μM | 149 ± 3.8 (41 ± 14) | 109 ± 3.0* (930 ± 240)# | 151 ± 4.5 (51 ± 6.9) |
0.5 μM | 262 ± 7.3 (3900 ± 690) | 161 ± 7.5‡ (7400 ± 1900) | 253 ± 4.7 (5030 ± 453) |
2.0 μM | 305 ± 2.3 (49 ± 1.5)† | 185 ± 6.7* (175 ± 35) | 271 ± 5.3 (311 ± 30) |
*p ≤ 0.001 and ‡p ≤ 0.01 when the maximal adenylyl cyclase superactivation levels in cells treated with etorphine were compared with those in cells treated with morphine and methadone using ANOVA.
↵# p ≤ 0.006 when the EC50 value of naloxone to elicit adenylyl cyclase superactivation in etorphine-treated cells was compared with that of morphine and methadone.
↵† p ≤ 0.001 when the EC50 value of naloxone to elicit adenylyl cyclase superactivation in cells treated with morphine was compared with that in cells treated with etorphine or methadone using ANOVA.