Kinetic parameters of 125I-MT7ox dissociation from hM1 receptor induced by MT7 toxin alone or in combination with various orthosteric and allosteric ligands.
CHO hM1 membranes were first preincubated for 18 to 20 h with 20 pM 125I-MT7ox, and then dissociation was initiated by 100 nM MT7 toxin with or without saturating concentrations of orthosteric or allosteric compounds. Koff values are the mean ± S.E.M. of three to five experiments.